The aim of this study was to examine the effect of a physical literacy and differential learning program in motor, technical and tactical basketball skills. Seventy-six college students (age 20.4 ± 1.9) were randomly allocated into control and experimental (BasketCAL) groups. The motor skills were assessed using Illinois Agility Test, technical abilities were evaluated through Taco Bell skills challenge and tactical variables were examined during a 4-on-4 full-court basketball game. Globally, the learning program and respective classes could be characterised by attentional breadth, large unpredictability and adaptation demands. The results indicated that BasketCAL group had a significant improvement in agility comparatively to the control group. Also, was noted that BasketCAL students decreased the unsuccessful actions performed during the game. Overall, this program prepares the players to attune the relevant stimulus through the development of adaptive behaviours to overcome environment constraints, leading to better game decisions.